Journal
BIOENGINEERED
Volume 9, Issue 1, Pages 116-123Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2017.1323592
Keywords
Acetate assimilation; aeration; mevalonate; two-stage fed-batch; Escherichia coli
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Funding
- National Natural Science Foundation of China [21572242, 21376255]
- Taishan Scholars Climbing Program of Shandong [tspd20150210]
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We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06g L-1 mevalonate with a productivity of 0.03g L-1 h(-1) in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7.85g L-1 and 0.13g L-1 h(-1), respectively. Fed-batch fermentation was further optimized under anaerobic and microaerobic conditions, and mevalonate concentrations reached 3.05g L-1 and 4.97g L-1, respectively, indicating that the oxygen supply exerts a large impact on mevalonate production from acetate. This study describes a method with high potential to produce mevalonate with the engineered E. coli strain XU143 using the less costly alternative feedstock acetate as a carbon source.
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