Journal
JOURNAL OF SEED SCIENCE
Volume 38, Issue 3, Pages 254-258Publisher
ABRATES-ASSOC BRASILEIRA TECHNOLOGIA SEMENTES
DOI: 10.1590/2317-1545v38n3161129
Keywords
cell viability; transmission electron microscopy; TUNEL test
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Funding
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) [PPM-00470-14]
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During germination, orthodox seeds lose their ability to tolerate desiccation resembling recalcitrant seeds. This research aimed to investigate the cell changes during the re-induction of the desiccation tolerance (DT) in Sesbania virgata germinated seeds with 1, 3 and 5 mm long radicles. To re-establish DT, germinated seeds were incubated for 72 h in polyethylene glycol (PEG, -2.04 MPa) before dehydration in silica gel (at 10% moisture content) followed by rehydration. Cell viability was assessed through TUNEL test in dry radicles and transmission electron microscopy in both fresh and dry radicles. The positive-TUNEL confirmed the DNA degradation, through the green fluorescence of the cell nuclei from 5 mm radicle length and the ultra structural evaluations detected loss of cellular content integrity in 3 and 5 mm cell radicles that did not survive dehydration to 10%.
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