Journal
ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY
Volume 73, Issue -, Pages 496-502Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2059798316019276
Keywords
RELION; cryo-EM; single-particle analysis
Funding
- UK Medical Research Council [MC_UP_A025_1013]
- MRC [MC_UP_A025_1013] Funding Source: UKRI
- Medical Research Council [MC_UP_A025_1013] Funding Source: researchfish
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The formal concept of a workflow to single-particle analysis of cryo-electron microscopy (cryo-EM) images in the RELION program is described. In this approach, the structure-determination process is considered as a graph, where intermediate results in the form of images or metadata are the vertices, and different functionalities of the program are the edges. The new implementation automatically logs all user actions, facilitates file management and disk cleaning, and allows convenient browsing of the history of a project. Moreover, new functionality to iteratively execute consecutive jobs allows on-the-fly image processing, which will lead to more efficient data acquisition by providing faster feedback on data quality. The possibility of exchanging data-processing procedures among users will contribute to the development of standardized image-processing procedures, and hence increase accessibility for new users in this rapidly expanding field.
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