Journal
3 BIOTECH
Volume 7, Issue -, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-017-0825-3
Keywords
Lycopene; Tomato peels; Antioxidant; Pectinase; Tri-solvent extraction
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Funding
- Department of Food Technology, University Institute of Chemical Technology, North Maharashtra University, Jalgaon, India
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In the present work, optimization of pectinaseassisted and tri-solvent-mediated extraction of lycopene from waste tomato peels was carried out. The optimized parameters for enzymatic pre-treatment were 2% pectinase concentration, pH 5.5, 4-h incubation, 45 degrees C and 150 rpm. Maximum recovery of lycopene from tomato peels using optimized tri-solvent extraction was achieved at 45 degrees C, 120-min incubation and 200 rpm. The extracted lycopene was confirmed through functional and characteristic peaks in UV-Vis and FTIR spectra and with retention time in HPLC. The radical scavenging activity was 72.30 +/- 2.70 and 43.40 +/- 2.01 mu g ascorbic acid equivalents (AAE)/ml for 1,1-diphenyl-2-picrylhydrzyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, respectively. The optimized method resulted in 7.38, 4.65 and 1.59 times enhancement in lycopene extraction and recovery in correlation with single solvent, enzyme-treated and tri-solvent extraction methods, respectively.
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