Journal
3 BIOTECH
Volume 8, Issue -, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-017-1041-x
Keywords
Trichoderma orientalis; Xylanase; beta-xylosidase; beta-xyl1 gene; Xylooligosaccharides; Xylose
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Funding
- National Natural Science Foundation of China [31170067, 31600475, 21303142]
- Jiangxi Province Science Foundation for Youths [20161BAB214177]
- Natural Science Foundation of Guangdong Province [2016A030310124]
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The goal of this study was to enhance the production of xylooligosaccharides (XOs) and reduce the production of xylose. We investigated beta-xylosidases, which were key enzymes in the hydrolysis of xylan into xylose, in Trichoderma orientalis EU7-22. The binary vector pUR5750G/bxl::hph was constructed to knock out the beta-xyl1 gene (encoding beta-xylosidases) in T. orientalis EU7-22 by homologous integration, producing the mutant strain T. orientalis Bxyl-1. Xylanase activity for strain Bxyl-1 was 452.42 IU/mL, which increased by only 0.07% compared to that of parental strain EU7-22, whereas beta-xylosidase activity was 0.06 IU/mL, representing a 91.89% decrease. When xylanase (200 IU/g xylan), produced by T. orientalis EU7-22 and T. orientalis Bxyl-1, was used to hydrolyze beechwood xylan, in contrast to the parental strain, the XOs were enhanced by 83.27%, whereas xylose decreased by 45.80% after 36 h in T. orientalis Bxyl-1. Based on these results, T. orientalis Bxyl-1 has great potential for application in the production of XOs from lignocellulosic biomass.
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