Journal
STEM CELL REPORTS
Volume 8, Issue 6, Pages 1757-1769Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2017.05.011
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Funding
- Paul G. Allen Family Foundation
- JPB Foundation
- Leona M. and Harry B. Helmsley Charitable Trust [2012-PG-MED002]
- Annette C. Merle-Smith [R01 MH095741, U19MH106434]
- G. Harold & Leila Y. Mathers Foundation
- Flow Cytometry Core Facility of the Salk Institute
- NIH-NCI CCSG [P30 014195]
- Next Generation Sequencing Core Facility of the Salk Institute
- Chapman Foundation
- Helmsley Charitable Trust
- Razavi Newman Integrative Genomics and Bioinformatics Core Facility of the Salk Institute
- Swiss-NSF outgoing PD fellowship
- Lynn and Edward Streim fellowship
- EMBO long-term fellowship
- Bettencourt Schueller Foundation
- Philippe Foundation
- Bob and Mary Jane Engman
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Astrocyte dysfunction and neuroinflammation are detrimental features in multiple pathologies of the CNS. Therefore, the development of methods that produce functional human astrocytes represents an advance in the study of neurological diseases. Here we report an efficient method for inflammation-responsive astrocyte generation from induced pluripotent stem cells (iPSCs) and embryonic stem cells. This protocol uses an intermediate glial progenitor stage and generates functional astrocytes that show levels of glutamate uptake and calcium activation comparable with those observed in human primary astrocytes. Stimulation of stem cell-derived astrocytes with interleukin-1 beta or tumor necrosis factor a elicits a strong and rapid pro-inflammatory response. RNA-sequencing transcriptome profiling confirmed that similar gene expression changes occurred in iPSC-derived and primary astrocytes upon stimulation with interleukin-1 beta. This protocol represents an important tool for modeling in-a-dish neurological diseases with an inflammatory component, allowing for the investigation of the role of diseased astrocytes in neuronal degeneration.
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