Journal
STEM CELL REPORTS
Volume 8, Issue 6, Pages 1563-1572Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2017.04.007
Keywords
-
Categories
Funding
- NIH: [NHLBI UO1 HL100395, Ancillary HL100395-07, 10560, Ancillary HL099997, NIAID R56 AI110831, NIAID R01 AI121197]
- Alex's Lemonade Stand Foundation
- Hyundai Hope on Wheels Foundation
Ask authors/readers for more resources
Recent evidence points to the embryonic emergence of some tissue-resident innate immune cells, such as B-1a lymphocytes, prior to and independently of hematopoietic stem cells (HSCs). However, whether the full hematopoietic repertoire of embryonic HSCs initially includes these unique lineages of innate immune cells has been difficult to assess due to lack of clonal assays that identify and assess HSC precursor (pre-HSC) potential. Here, by combining index sorting of single embryonic hemogenic precursors with in vitro HSC maturation and transplantation assays, we analyze emerging pre-HSCs at the single-cell level, revealing their unique stage-specific properties and clonal lineage potential. Remarkably, clonal pre-HSCs detected between E9.5 and E11.5 contribute to the complete B cell repertoire, including B-1a lymphocytes, revealing a previously unappreciated common precursor for all B cell lineages at the pre-HSC stage and a second embryonic origin for B-1a lymphocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available