Journal
STEM CELL REPORTS
Volume 8, Issue 1, Pages 30-38Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2016.11.012
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Funding
- Agency for Innovation by Science and Technology (IWT) [SB/111245]
- Willy Gepts Fund of UZ Brussel [WFWG15-01]
- Methusalem (METH3)
- Scientific Research Foundation Flanders (FWO) [V434114N]
- FWO-Kom Op Tegen Kanker (KOTK) [G.0E39.14N]
- Vrije Universiteit Brussel [BAS50]
- Swedish Research Council/Finnish Academy of Science [2012-6352]
- Emil and Wera Cornell Foundation
- Jeansson Foundation [JS2014-0091]
- Swedish Childhood Cancer Foundation [TJ2016-0093]
- Sallskapet Barnavard
- regional agreement on medical training and clinical research (ALF)
- [EU-FP7-PEOPLE-2013-ITN603568]
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So far, successful de novo formation of testicular tissue followed by complete spermatogenesis in vitro has been achieved only in rodents. Our findings reveal that primary human testicular cells are able to self-organize into human testicular organoids (TOs), i. e., multi-cellular tissue surrogates, either with or without support of a biological scaffold. Despite lacking testis-specific topography, these mini-tissues harbored spermatogonia and their important niche cells, which retained specific functionalities during long-term culture. These observations indicate the posibility of in vitro re-engineering of a human testicular microenvironment from primary cells. Human TOs might help in the development of a biomimetic testicular model that would exert a tremendous impact on research and development, clinical treatment of infertility, and screening in connection with drug discovery and toxicology.
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