Journal
STEM CELL REPORTS
Volume 8, Issue 2, Pages 387-400Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2017.01.004
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Funding
- National Institute of Arthritis and Musculoskeletal and Skin Diseases of the NIH [R01 AR064803]
- University of Wisconsin-Madison Carbone Cancer Center [P30 CA014520]
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Maintaining properties of human bone marrow-derived mesenchymal stem cells (BMSCs) in culture for regenerative applications re-mains a great challenge. An emerging approach of constructing a culture environment mimicking the bone marrow niche to regulate BMSC activities has been developed. In this study, we have demonstrated a systematic approach to identify soluble factors of interest extracted from human bone marrow and used them in BMSC culture for tissue regeneration. We have found that lipocalin-2 and prolac-tin are key factors in bone marrow, involved in regulating BMSC activities. Treating the cell with lipocalin-2 and prolactin delays cellular senescence of BMSCs and primes the cell for osteogenesis and chondrogenesis. We have also demonstrated that BMSCs pretreated with lipocalin-2 and prolactin can enhance the repair of calvarial defects in mice. Together, our study provides research evidence of using a viable approach to prime BMSC properties in vitro for improving cell-based tissue regeneration in vivo.
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