Journal
STEM CELL REPORTS
Volume 9, Issue 5, Pages 1432-1440Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2017.09.002
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Funding
- Max-Planck Graduate Center during her PhD program
- Red de excelencia Consolider-Ingenio Spanish Ion Channel Initiative [BFU2015-70067REDC]
- MEC [BFU2014-53654-P]
- BRADECM [S2013/ICE-2958]
- UCM-Santander [PR26/16-18B-3]
- Fundacion Ramon Areces [PR2018/16-02]
- Ramon y Cajal Program of the Spanish Ministry of Economy and Competitiveness (MEC) [RYC-2013-13290]
- China Scholarship Council
- Human Frontier Science Program Long-Term Fellowship
- NEURON ERA-NET grant [01EW1604]
- Johannes Gutenberg University Mainz
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Engineering of biomaterials with specific biological properties has gained momentum as a means to control stem cell behavior. Here, we address the effect of bifunctionalized hydrogels comprising polylysine (PL) and a 19-mer peptide containing the laminin motif IKVAV (IKVAV) on embryonic and adult neuronal progenitor cells under different stiffness regimes. Neuronal differentiation of embryonic and adult neural progenitors was accelerated by adjusting the gel stiffness to 2 kPa and 20 kPa, respectively. While gels containing IKVAV or PL alone failed to support long-term cell adhesion, in bifunctional gels, IKVAV synergized with PL to promote differentiation and formation of focal adhesions containing beta(1)-integrin in embryonic cortical neurons. Furthermore, in adult neural stem cell culture, bifunctionalized gels promoted neurogenesis via the expansion of neurogenic clones. These data highlight the potential of synthetic matrices to steer stem and progenitor cell behavior via defined mechano-adhesive properties.
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