Journal
STEM CELL REPORTS
Volume 9, Issue 6, Pages 1898-1915Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2017.10.018
Keywords
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Funding
- Medical Research Council UK [MR/M015688/1, MR/M007871/1, MR/J004553/1]
- New Life
- Fight for Sight
- Rosetrees Trust
- National Institute for Health Research (NIHR) Great Ormond Street Hospital Biomedical Research Center (GOSH BRC)
- NIHR Moor-fields Biomedical Research Center
- RP Fighting Blindness [GR576]
- European Research Council [2012-ADG_20120314]
- Great Ormond Street Hospital Children's Charity
- MRC [MR/J004553/1, G0901550, MR/L012758/1, MR/M015688/1, MR/M007871/1] Funding Source: UKRI
- Cancer Research UK [22231] Funding Source: researchfish
- Fight for Sight [1532/33] Funding Source: researchfish
- Great Ormond Street Hospital Childrens Charity [V2816] Funding Source: researchfish
- Medical Research Council [MR/M007871/1, G0901550, MR/L012758/1, MR/J004553/1, MR/M015688/1] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0508-10130, NF-SI-0515-10069, NF-SI-0513-10074] Funding Source: researchfish
- Rosetrees Trust [M257] Funding Source: researchfish
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Loss of cone photoreceptors, crucial for daylight vision, has the greatest impact on sight in retinal degeneration. Transplantation of stem cell-derived L/M-opsin cones, which form 90% of the human cone population, could provide a feasible therapy to restore vision. However, transcriptomic similarities between fetal and stem cell-derived cones remain to be defined, in addition to development of cone cell purification strategies. Here, we report an analysis of the human L/M-opsin cone photoreceptor transcriptome using an AAV2/9. pR2.1:GFP reporter. This led to the identification of a cone-enriched gene signature, which we used to demonstrate similar gene expression between fetal and stem cell-derived cones. We then defined a cluster of differentiation marker combination that, when used for cell sorting, significantly enriches for cone photoreceptors from the fetal retina and stem cell-derived retinal organoids, respectively. These data may facilitate more efficient isolation of human stem cell-derived cones for use in clinical transplantation studies.
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