Journal
EUROPEAN POLYMER JOURNAL
Volume 69, Issue -, Pages 532-539Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.eurpolymj.2015.01.043
Keywords
Multiply responsive; Vault; Nanoparticle; Conjugate
Categories
Funding
- National Institutes of Health [R21 CA 137506-01]
- National Science Foundation [DGE-0707424]
- UCLA
- NIH [1S10RR23057]
- CNSI at UCLA
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Multiply responsive protein nanoparticles are interesting for a variety of applications. Herein, we describe the synthesis of a vault nanoparticle that responds to both temperature and pH. Specifically, poly(N-isopropylacrylamide-co-acrylic acid) with a pyridyl disulfide end group was prepared by reversible addition-fragmentation chain transfer (RAFT) polymerization. The polymer had a lower critical solution temperature (LCST) of 31.9 degrees C at pH 5, 44.0 degrees C at pH 6 and above 60 degrees C at pH 7. The polymer was conjugated to human major vault protein (hMVP), and the resulting nanoparticle was analyzed by UV-Vis, dynamic light scattering (DLS) and electron microscopy. The data demonstrated that the poly(N-isopropylacrylamide-co-acrylic acid)-vault conjugate did not respond to temperatures below 60 degrees C at pH 7, while the nanoparticles reversibly aggregated at pH 6. Furthermore, it was shown that the vault nanoparticle structure remained intact for at least three heat and cooling cycles. Thus, these dually responsive nanoparticles may serve as a platform for drug delivery and other applications. (C) 2015 Elsevier Ltd. All rights reserved.
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