Journal
NANOMATERIALS
Volume 7, Issue 2, Pages -Publisher
MDPI AG
DOI: 10.3390/nano7020028
Keywords
magnetic nanoparticles; transfection; SH-SY5Y; hippocampal neurons; cortical neurons; non-viral gene delivery; nanomagnetic gene transfection; biomagnetics; gradient magnetic field; permanent magnets
Categories
Funding
- nanoTherics limited
- EU-ITN network Mag(net)icFun [PITN-GA-2012-290248]
- EPSRC [1979819] Funding Source: UKRI
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To develop treatments for neurodegenerative disorders, it is critical to understand the biology and function of neurons in both normal and diseased states. Molecular studies of neurons involve the delivery of small biomolecules into cultured neurons via transfection to study genetic variants. However, as cultured primary neurons are sensitive to temperature change, stress, and shifts in pH, these factors make biomolecule delivery difficult, particularly non-viral delivery. Herein we used oscillating nanomagnetic gene transfection to successfully transfect SH-SY5Y cells as well as primary hippocampal and cortical neurons on different days in vitro. This novel technique has been used to effectively deliver genetic material into various cell types, resulting in high transfection efficiency and viability. From these observations and other related studies, we suggest that oscillating nanomagnetic gene transfection is an effective method for gene delivery into hard-to-transfect neuronal cell types.
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