Journal
MICROBIOLOGYOPEN
Volume 7, Issue 2, Pages -Publisher
WILEY
DOI: 10.1002/mbo3.551
Keywords
adhesion; environmental factors; RNAi; secA; secD; secF; Vibrio alginolyticus; yajC; yidC
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Funding
- National Natural Science Foundation of China [31672694, 31702384]
- Science and Technology Major
- Special Project of Fujian Province [2016NZ0001-3]
- Fujian Provincial Department of Science Technology [JA15289]
- Natural Science Foundation of Fujian Province [2016J05080]
- Scientific Research Fund of Fujian Provincial Department of Education [JA15292]
- Discipline construction fund of li shangda of Jimei University [C515044]
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Vibrio alginolyticus caused great losses to aquaculture. Adhesion is an important virulence factor of V. alginolyticus. In this study, the relationship between V. alginolyticus adhesion and type II secretion system genes (secA, secD, secF, yajC, and yidC) was determined using gene silencing, qRT-PCR and in vitro adhesion assay. The results showed that the expression of target genes and the bacterial adhesion exhibited significant decreases after transient gene silencing and stable gene silencing, which indicated that secA, secD, secF, yajC, and yidC played roles in the bacterial adhesion of V. alginolyticus. The expression of secA, secD, secF, yajC, and yidC were significantly influenced by temperature, salinity, pH and starvation. The results indicated that the expression of secA, secD, secF, yajC, and yidC were sensitive to different environmental factors, whereas environmental factors can affect V. alginolyticus adhesion via the expression of secA, secD, secF, yajC, and yidC.
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