Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 130, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/55641
Keywords
Developmental Biology; Issue 130; Rotary culture system; three-dimensional culture; simulated microgravity; ovarian follicle; alginate; in vitro
Categories
Funding
- Natural Science Foundation of Zhejiang Province [LY13C120002]
Ask authors/readers for more resources
14 day-old mouse ovarian tissue and preantral follicles isolated from same-aged mice were incubated in a simulated microgravity culture system. We quantitatively assessed follicle survival, measured follicle and oocyte diameters, and examined ultrastructure of the oocytes produced from the system. We observed decreased follicle survival, downregulation of expressions of proliferating cell nuclear antigen and growth differentiation factor 9, as indicators for the development of granulosa cells and oocytes, respectively, and oocyte ultrastructural abnormalities under the simulated microgravity condition. The simulated microgravity experimental setup needs to be optimized to provide a model for investigation of the mechanisms involved in the oocyte/follicle in vitro development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available