Journal
PHOTONICS
Volume 3, Issue 4, Pages -Publisher
MDPI
DOI: 10.3390/photonics3040056
Keywords
single-photon avalanche diode; time-correlated single-photon counting; confocal microscopy; time-resolved imaging; high-content screening; drug discovery
Categories
Funding
- Ontario Centres of Excellence through a Market Readiness grant
- Natural Sciences and Engineering Research Council of Canada through an Idea to Innovation Grant
- Industry Postgraduate PhD Scholarship (AT)
- Canadian Foundation of Innovation
Ask authors/readers for more resources
Current instruments used to detect specific protein-protein interactions in live cells for applications in high-content screening (HCS) are limited by the time required to measure the lifetime. Here, a 32 x 1 single-photon avalanche diode (SPAD) array was explored as a detector for fluorescence lifetime imaging (FLIM) in HCS. Device parameters and characterization results were interpreted in the context of the application to determine if the SPAD array could satisfy the requirements of HCS-FLIM. Fluorescence lifetime measurements were performed using a known fluorescence standard; and the recovered fluorescence lifetime matched literature reported values. The design of a theoretical 32 x 32 SPAD array was also considered as a detector for a multi-point confocal scanning microscope.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available