The effect of types and concentrations of auxins on callus induction of Centella asiatica

Rahayu S, Ika Roostika I, Bermawie N. 2016. The effect of types and concentrations of auxins on callus induction of Centella asiatica. Nusantara Bioscience 8: 283-287. Centella (Centella asiatica L.) is one of medicical crops that have various utility as healing wounds, inflammation, rheumatic, hemorrhoids, tuberculosis, dysentry, leprosy, and fever and also as appetizer. In recent years the demand of raw materials of medicinal from centella have increased. Until now, centella has been harvested directly from nature without replanting so that this plant was worried to be extinct gradually. In vitro culture is one of the techniques for plant propagation. This technique have proved to be able to propagate a plant in large numbers, short time and uniform. Callus induction is initial stages of plant propagation by indirect organogenesis and somatic embryogenesis. The objectives of this research were to find out the most suitable type of auxins and its concentrations to induce callus formation of centella. Explants used in this research were leaves and petioles taken from sterille culture of centella var. Castina 1. Three kinds of auxins were used to this research i. e. 2,4-D, Picloram and Dicamba at the levels of 2, 4 and 6 mgL(-1). The result indicated that the most suitable type of auxin for inducing callus formation was 2,4-D or Dicamba at concentration of 4 mgL(-1) which gave the highest percentage of callus formation, friable-callus texture and the highest fresh weight as well.