Journal
GENES
Volume 8, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/genes8110317
Keywords
Toxicodendron radicans; poison ivy; urushiol; contact dermatitis; skin rash; transcriptome; Anacardiaceae
Categories
Funding
- Virginia Bioinformatics Institute Core Lab
- Fralin Life Science Institute Next-Gen DNA sequencing grant
- Virginia Agricultural Experiment Station
- Molecular/Translational Plant Science graduate student research
- Plant Pathology, Physiology, and Weed Science department
- Genetics, Bioinformatics, and Computational Biology graduate program at Virginia Tech
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Contact with poison ivy plants is widely dreaded because they produce a natural product called urushiol that is responsible for allergenic contact delayed-dermatitis symptoms lasting for weeks. For this reason, the catchphrase most associated with poison ivy is leaves of three, let it be, which serves the purpose of both identification and an appeal for avoidance. Ironically, despite this notoriety, there is a dearth of specific knowledge about nearly all other aspects of poison ivy physiology and ecology. As a means of gaining a more molecular-oriented understanding of poison ivy physiology and ecology, Next Generation DNA sequencing technology was used to develop poison ivy root and leaf RNA-seq transcriptome resources. De novo assembled transcriptomes were analyzed to generate a core set of high quality expressed transcripts present in poison ivy tissue. The predicted protein sequences were evaluated for similarity to SwissProt homologs and InterProScan domains, as well as assigned both GO terms and KEGG annotations. Over 23,000 simple sequence repeats were identified in the transcriptome, and corresponding oligo nucleotide primer pairs were designed. A pan-transcriptome analysis of existing Anacardiaceae transcriptomes revealed conserved and unique transcripts among these species.
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