Journal
CHEMELECTROCHEM
Volume 4, Issue 4, Pages 864-871Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/celc.201600765
Keywords
adenosine-5-triphosphate (ATP); poly (benzoxazine); immobilization; enzyme microbiosensor; live cell measurements
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Funding
- Boehringer Ingelheim Ulm University BioCenter (BIU) [D.5008]
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The reproducible immobilization of enzymes represents a key requirement in developing sensitive and fast-responding amperometric microbiosensors. A microbiosensor for the respective detection of glucose and adenosine-5'-triphosphate (ATP) is presented by using a poly(benzoxazine) derivative for the entrapment of the enzymes glucose oxidase (GOD) and hexokinase (HEX) at platinum (Pt) microelectrodes (MEs). For glucose, a sensitivity of 123.05 +/- 10.78pA/mM (n=5) was obtained, which shows twice as high sensitivity compared to microbiosensors that use electrophoretic paints as the immobilization matrix for the same size ME (radius: 25m). For the determination of ATP, a sensitivity of 48.47 +/- 5.12pA/mu M and a signal-to-noise ratio of 40 at physiological pH values were obtained. Apart from their enhanced sensitivity, a significant improvement of these sensors is related to their improved mechanical stability. The applicability of these poly(benzoxine)-based microbiosensors for ATP detection was demonstrated with measurements at receptor protein tyrosine phosphatase zeta (PTPR) osteoblastic cells during mechanical stimulation.
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