Journal
APPLICATIONS IN PLANT SCIENCES
Volume 5, Issue 7, Pages -Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/apps.1700023
Keywords
Fabaceae; Guibourtia ehie; microsatellites; next-generation sequencing
Categories
Funding
- Fonds pour la Formation a la Recherche dans l'Industrie et l'Agriculture (FRIA)
- Fonds de la Recherche Scientifique (F.R.S.-FNRS) [T.0163.13]
- Belgian Science Policy (AFRIFORD project)
- Fonds Francais pour l'Environnement Mondial (FFEM)
- CEB Precious Woods
- Wijma Cameroun S.A.
- Centre national de la recherche scientifique et technique (CENAREST)
Ask authors/readers for more resources
Premise of the study: Microsatellite primers (simple sequence repeats [SSRs]) were developed in Guibourtia ehie (Fabaceae, Detarioideae) to study population genetic structure and the history of African vegetation. Methods and Results: We isolated 18 polymorphic SSRs from a nonenriched genomic library. This set of primer pairs was tested on four populations, and the results showed two to 16 alleles per locus with mean observed and expected heterozygosities of 0.27 0.05 and 0.57 +/- 0.05, respectively. Cross-amplification tests in 13 congeneric species were successful for the four taxa belonging to the subgenus Gorskia. Conclusions: This set of microsatellite markers will be useful to investigate the phylogeography and population genetics of G. ehie, a key representative of African semideciduous moist forests.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
