Journal
SCIENCE SIGNALING
Volume 10, Issue 502, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/scisignal.aam7464
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Funding
- NIH [CA088932, CA173687, DE016572, P01 CA203628, C06 RR015455]
- Hollings Cancer Center [P30 CA138313]
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Signaling by the transforming growth factor-beta (TGF-beta) receptors I and II (T beta RI/II) and the primary cilia-localized sonic hedgehog (Shh) pathway promote cell migration and, consequently, tumor metastasis. In contrast, the sphingolipid ceramide inhibits cell proliferation and tumor metastasis. We investigated whether ceramide metabolism inhibited T beta RI/II trafficking to primary cilia to attenuate cross-talk between T beta RI/II and the Shh pathway. We found that ceramide synthase 4 (CerS4)-generated ceramide stabilized the association between T beta RI and the inhibitory factor Smad7, which limited the trafficking of T beta RI/II to primary cilia. Expression of a mutant T beta RI that signals but does not interact with Smad7 prevented the CerS4-mediated inhibition of migration in various cancer cells. Genetic deletion or knockdown of CerS4 prevented the formation of the Smad7-T beta RI inhibitory complex and increased the association between T beta RI and the transporter Arl6 through a previously unknown cilia-targeting signal (Ala(31)Thr(32)Ala(33)Leu(34)Gln(35)) in T beta RI. Mutating the cilia-targeting signal abolished the trafficking of T beta RI to the primary cilia. Localization of T beta RI to primary cilia activated a key mediator of Shh signaling, Smoothened (Smo), which stimulated cellular migration and invasion. T beta RI-Smo cross-talk at the cilia in CerS4-deficient 4T1 mammary cancer cells induced liver metastasis from orthotopic allografts in both wild-type and CerS4-deficient mice, which was prevented by overexpression of Smad7 or knockdown of intraflagellar transport protein 88 (IFT88). Overall, these data reveal a ceramide-dependent mechanism that suppresses cell migration and invasion by restricting T beta RI/II-Shh signaling selectively at the plasma membrane of the primary cilium.
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