Journal
NUCLEUS
Volume 8, Issue 5, Pages 506-514Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/19491034.2017.1337621
Keywords
chromatin; DNA repair; heterochromatin; lamina associated domains; lamin; mechanics; nuclear architecture; nuclear envelope; nuclear structures; nuclear transport; topography
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Funding
- Holcim Science Foundation
- Swiss National Science Foundation (SNSF) [CR32I3-156931]
- ETH Zurich
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Recent advances in fluorescence microscopy have opened up new possibilities to investigate chromosomal and nuclear 3D organization on the nanoscale. We here discuss their potential for elucidating topographical details of the nuclear lamina. Single molecule localization microscopy (SMLM) in combination with immunostainings of lamina proteins readily reveals tube-like invaginations with a diameter of 100-500nm. Although these invaginations have been established as a frequent and general feature of interphase nuclei across different cell types, their formation mechanism and function have remained largely elusive. We critically review the current state of research, propose possible connections to lamina associated domains (LADs), and revisit the discussion about the potential role of these invaginations for accelerating mRNA nuclear export. Illustrative studies using 3D super-resolution imaging are shown and will be instrumental to decipher the physiological role of these nanoscale invaginations.
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