Journal
MICROMACHINES
Volume 8, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/mi8030083
Keywords
cell lysis; cell lysis methods; microfluidics; electrical lysis; mechanical lysis; thermal lysis
Categories
Funding
- Ontario Research Fund - Research Excellence Program
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs Program
Ask authors/readers for more resources
The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available