Journal
MICROFABRICATED AND NANOFABRICATED SYSTEMS FOR MEMS/NEMS 12
Volume 75, Issue 17, Pages 11-17Publisher
ELECTROCHEMICAL SOC INC
DOI: 10.1149/07517.0011ecst
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Exosomes contain disease biomarkers for cancer and other pathological conditions. They are groups of nano-scale extracellular communication organelles which dynamically transport cargoes of molecules and genetic materials between cells. Exosomes circulating in our body-fluids provide a snap-shot in real-time for virtually all aspects of our physiology. Isolation detection and quantification methods of exosomes from various bio-fluids are challenging for clinical applications, like liquid biopsy. This paper presents a simple label-free technique to capture and detect exosomes integrating an exosome-capturing synthetic polypeptide (called Vn96). This exosome-capture can efficiently quantitate exosomes present in a fluid and will be used for subsequent molecular analysis (protein and nucleic acid) to facilitate liquid biopsy.
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