Journal
CARBOHYDRATE POLYMERS
Volume 155, Issue -, Pages 117-127Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2016.08.056
Keywords
Grafting; Modified chitosan; Epigallocatechin gallate; Antibacterial; Antioxidant
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Funding
- Division de Ciencias Biologicas y de la Salud of Universidad de Sonora [DCBS15-USO313002192]
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Chitosan was functionalized with epigallocatechin gallate (EGCG) by a free radical-induced grafting procedure, which was carried out by a redox pair (ascorbic acid/hydrogen peroxide) as the radical initiator. The successful preparation of EGCG grafted-chitosan was verified by spectroscopic (UV, FTIR and XPS) and thermal (DSC and TGA) analyses. The degree of grafting of phenolic compounds onto the chitosan was determined by the Folin-Ciocalteu procedure. Additionally, the biological activities (antioxidant and antibacterial) of pure EGCG, blank chitosan and EGCG grafted-chitosan were evaluated. The spectroscopic and thermal results indicate chitosan functionalization with EGCG; the EGCG content was 25.8 mg/g of EGCG grafted-chitosan. The antibacterial activity of the EGCG grafted-chitosan was increased compared to pure EGCG or blank chitosan against S. aureus and Pseudomonas sp. (p<0.05). Additionally, EGCG grafted-chitosan showed higher antioxidant activity than blank chitosan. These results indicate that EGCG grafted-chitosan might be useful in active food packaging. (C) 2016 Elsevier Ltd. All rights reserved.
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