Journal
APPLIED SURFACE SCIENCE
Volume 392, Issue -, Pages 883-888Publisher
ELSEVIER
DOI: 10.1016/j.apsusc.2016.09.111
Keywords
SU-8; Photolithography; Photoluminescence; Immunosensing
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Funding
- Spanish Ministry of Economy and Competitiveness MINECO [TEC2015-71324-R]
- Catalan Government under project AGAUR [SGR 1344]
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SU-8, an epoxy based negative photoresist is extensively used as a structural material for the fabrication of microelectro-mechanical systems and in microelectronics technology. However, the possible applications of SU-8 for biosensing have not been explored much, mainly because of the photoluminescence SU-8 possesses in the near-UV and visible wavelength ranges which hinders fluorescent labelling of biorecognition events. In this study we demonstrate that photoluminescence of SU-8 can be employed itself as a sensing transduction parameter to produce a tool for immunosensing: the photoluminescence shows a systematic reduction upon modification of its surface chemistry, and in particular upon attachment of an antigen-antibody (algG-IgG) pair. We investigate the relation of the amount of reduction of photoluminescence on planar and microstructured surfaces, and we show that microstructuring leads to a higher reduction than a planar surface. Furthermore, we evaluated the dependence of photoluminescence reduction as a function of analyte concentration to prove that this magnitude can be applied to immunosensing. (C) 2016 Elsevier B.V. All rights reserved.
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