Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 5, Issue 43, Pages 8532-8541Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7tb02114c
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Funding
- National Natural Science Foundation of China [31771026, 51403158, 81771984, 21601139]
- National Key RD Program [2016YFC1101201]
- Zhejiang Natural Science Foundation of China [21601139]
- Science & Technology Program of Wenzhou [Y20160068, Y20160058, Y20160061]
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The adhesion of bacteria is one of the most important stages in biofilm formation and bacterial infection. Once bacteria have adhered to a biomaterial surface, it is hard to eliminate them, and bacterial growth and infection are inevitable. In the current study, we have designed and constructed enzymatically degradable (hyaluronic acid/chitosan)(n)-(hyaluronic acid/polylysine)(n) ((HA/CHI)(n)-(HA/PLL)(n)) composite multilayer films via a layer-by-layer self-assembly method. Spectroscopic ellipsometry and scanning electron microscopy cross section measurements showed the exponential growth behavior of (HA/CHI) 10-(HA/PLL)(10) multilayer films (similar to 2 mu m). The increased secretion of hyaluronidase and chymotrypsin in the bacterial infection microenvironment led to the fast degradation of the outer (PLL/HA)(n) multilayer films in the first 24 h. Enzymatic degradation of the multilayer films efficiently reduced the adhesion of both Staphylococcus aureus and Escherichia coli (499%). Bacterial live/dead staining demonstrated the bactericidal action of the remaining bottom (CHI/HA)(n) multilayer films against the two kinds of bacteria. In vivo subcutaneous tests on New Zealand white rabbits, wound appearance and histopathology analysis showed that the implantation of composite multilayer film-modified PDMS promoted wound healing and the materials demonstrated a self-defense antibacterial effect. The material demonstrated both anti-adhesive and bactericidal properties and could be used to modify biomedical implants.
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