Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 5, Issue 32, Pages 6681-6687Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7tb01557g
Keywords
-
Categories
Funding
- Barrie de la Maza Foundation
- Fondazione Cariverona
- Verona Nanomedicine Initiative
- Italian Ministry of Health [RF-2010-2305526]
Ask authors/readers for more resources
Prostate-specific membrane antigen (PSMA), a glycoprotein expressed in the prostatic epithelium endowed with enzymatic activity, is a very promising diagnostic marker for the early detection of prostate cancer. In this study, we report a novel electrochemiluminescence ELISA-like immunosensor based on carbon nanotubes and a highly specific sandwich immunoassay for the PSMA detection. To fabricate the device, an optically transparent electrode was modified with doubly functionalized multi-walled carbon nanotubes carrying amine groups and a monoclonal anti-PSMA antibody. Subsequently, to complete the sandwich immunosensing device, a second specific monoclonal anti-PSMA antibody was labelled with a electrochemiluminescent probe. Under optimized experimental conditions, the proposed sensing device exhibits a performance exceeding that of the state of-the-art in terms of the limit of detection (LOD) and limit of quantification (LOQ) as good as 0.88 ng mL(-1) and 2.60 ng mL(-1), respectively, in real complex samples such as cell lysates. In addition, the unique role of carbon nanotubes is also discussed by comparison with an analogue sensor assembled without the nanocarbon-based material.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available