Journal
IMMUNE SYNAPSE: Methods and Protocols
Volume 1584, Issue -, Pages 497-515Publisher
HUMANA PRESS INC
DOI: 10.1007/978-1-4939-6881-7_31
Keywords
Immunological synapse; MTOC; Lytic granules; Convergence; Confocal microscopy; Mathematical algorithm
Categories
Funding
- NIH [R01AI069746, R01AI120989]
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Natural killer (NK) cells contain specialized lysosome-related organelles termed lytic granules allowing them to mediate cytotoxicity against tumorigenic or virally infected target cells. NK cells polarize their lytic granules toward a target cell via the microtubule-organizing center (MTOC). Prior to that, however, lytic granules converge to the MTOC along microtubules utilizing minus-end-directed microtubule motors. Herein we describe how to visualize and quantify lytic granule convergence using confocal microscopy to gain quantitative insight into NK cell cytotoxicity and its regulation.
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