Journal
COGENT FOOD & AGRICULTURE
Volume 3, Issue 1, Pages -Publisher
TAYLOR & FRANCIS AS
DOI: 10.1080/23311932.2017.1419788
Keywords
Aflatoxin; Ochratoxin A; photochemical reaction device; HPLC-FLD; immunoaffinity column
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3A sensitive and robust HPLC method with fluorescence detector (FLD), photochemical reaction device (PHRED) was developed to determine Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in animal feeds. Immunoaffinity columns (IAC) was used to purify the samples. According to monitoring resulted with the established method, aflatoxin and ochratoxin A were found in 44 of 496 domestic commercial samples (8.9% detection rate) and the concentration ranges were 1.76-162.69 mu g/kg for Aflatoxin B1, 23.01-45.42 mu g/kg for Ochratoxin A in 2015. In 2016, % detection rate was 22.2% (115 of 518 samples) and the concentration ranges are 1.76-43.69 mu g/kg for Aflatoxin B1, 3.38-15.80 mu g/kg for Ochratoxin A. The developed method was specific and reliable and is suited for the routine analysis of aflatoxin in animal feed.
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