Analytical method development and monitoring of Aflatoxin B1, B2, G1, G2 and Ochratoxin A in animal feed using HPLC with Fluorescence detector and photochemical reaction device

3A sensitive and robust HPLC method with fluorescence detector (FLD), photochemical reaction device (PHRED) was developed to determine Aflatoxins (B1, B2, G1, and G2) and Ochratoxin A in animal feeds. Immunoaffinity columns (IAC) was used to purify the samples. According to monitoring resulted with the established method, aflatoxin and ochratoxin A were found in 44 of 496 domestic commercial samples (8.9% detection rate) and the concentration ranges were 1.76-162.69 mu g/kg for Aflatoxin B1, 23.01-45.42 mu g/kg for Ochratoxin A in 2015. In 2016, % detection rate was 22.2% (115 of 518 samples) and the concentration ranges are 1.76-43.69 mu g/kg for Aflatoxin B1, 3.38-15.80 mu g/kg for Ochratoxin A. The developed method was specific and reliable and is suited for the routine analysis of aflatoxin in animal feed.