Journal
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
Volume 209, Issue -, Pages 18-24Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.aanat.2016.09.005
Keywords
Calcium; Connexin 43; Stem cell; Osteogenesis; Bone; Hemichannel gating mechanism
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Funding
- DFG/Collaborative Research Center [Transregio 79, TRR79-TP-B05]
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The effects of extracellular calcium on osteogenic differentiation capacity of human bone-derived mesenchymal stromal cells with special regard to connexin 43 (cx43) have been investigated by means of cell culture experiments. Mesenchymal stromal cells isolated from human cancellous bone were cultured on tissue culture plates at different calcium ion (Ca2+) concentrations (1.8 mmol l(-1), 10 mmol l(-1), 20 mmoll(-1)). Cell responses were evaluated by quantitative RT-PCR, immunofluorescence staining, and Lucifer Yellow fluorescence uptake experiments. It could be shown that increasing Ca2+ concentrations correlate with increasing cx43 and bone sialoprotein mRNA levels as well as with enhanced cx43 fluorescence signaling and matrix mineralization of the cultures as shown by von Kossa staining. Hemichannel gating - assessed by Lucifer Yellow uptake - increases with increasing extracellular Ca2+ concentrations suggesting that regulatory effects at the hemichannel level are calcium-dependent. (C) 2016 Elsevier GmbH. All rights reserved.
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