Rapid and Selective Determination of Folate Receptor alpha with Sensitive Resonance Rayleigh Scattering Signal

A rapid, simple, and novel method for folate receptor alpha (FR alpha) determination is reported here. A probe of gold nanoparticles (Au NPs) modified with anti-FR alpha antibody was synthesized under the optimized conditions first. The antibody-modified Au NPs would aggregate when FR alpha was added to the probe for the specific interaction between antibody and antigen, resulting in the enhancement of resonance Rayleigh scattering (RRS) intensity. There is a linear relationship between the change of RRS intensity (Lambda I-RRS) and the concentration of FR alpha, with the detecting range of 0.50-37.50 ng.mL(-1) and the limit of determination of 0.05 ng.mL(-1). The determination of FR alpha in serum samples was realized with the advantages of high selectivity, high sensitivity, and easy operation.