Journal
BIOSENSORS & BIOELECTRONICS
Volume 87, Issue -, Pages 772-778Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.08.098
Keywords
Carbon quantum dots; Gold nanoparticles; Arginine; Isoelectric point
Categories
Funding
- National Natural Science Foundation of China [21273174]
- Municipal Science Foundation of Chongqing City [CSTC-2013jjB00002]
Ask authors/readers for more resources
A bidimensional optical sensing platform which combines the advantages of fluorescence and colorimetry has been designed for arginine (Arg) detection. The system was established by monitoring the influence of Arg on the growth of gold nanoparticles/carbon quantum dots (Au/CQDs) composite, and the CQDs synthesized by ethylene glycol were used as the reducing and stabilizing agent in this paper. Considering that Arg is the only amino acid with guanidine group and has the highest isoelectric point (pI) value at 10.76, Arg would carry positive charges at pH 7.4. Consequently, the positively charged guanidine group of Arg could attract AuCl4- and CQDs through electrostatic interaction, which inhibited the growth of Au/CQDs composite. Thereby, the color of the system almost did not change and the fluorescence quenching of CQDs was prevented in the presence of Arg. Based on the color change a low detection limit for Arg was 37 nM, and a detection limit of 450 nM was obtained by fluorescence spectroscopy. Moreover, this dual-signal sensor also revealed excellent selectivity toward Arg over other amino acids. Besides, Arg can be detected in urine samples with satisfactory results, which demonstrate the potential applications for real analysis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available