Journal
BIOSENSORS & BIOELECTRONICS
Volume 87, Issue -, Pages 802-806Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2016.09.015
Keywords
Electrochemiluminescence; PtNi nanocube; ConA; Peroxydisulfate/oxygen system
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Funding
- National Natural Science Foundation of China [51473136, 21575116]
- Natural Science Foundation Project of Chongqing City [CSTC-2014JCYJA20005]
- Fundamental Research Funds for the Central Universities, China [XDJK2015A002, SWU115004]
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A sandwich-configuration electrochemiluminescence (ECL) biosensor was constructed for detecting concanavalin A (ConA) based on peroxydisulfate/oxygen (S2O82-/O-2) system. In this work, the gold nanoflower modified Zn-doped SnO2 was used as a substrate to adsorb recognition element horseradish peroxidase (HRP) for binding ConA. Then, Au nanoparticles-thiosemicarbazide functionalized PtNi nanbcubes (AuNPs-TSC-PtNi NCs), as a novel ECL signal tracer, were incubated onto the electrode through a specific carbohydrate-ConA interaction, thus achieving a sandwiched structure. The integration of amplifying effect of both TSC and PtNi NCs on the ECL of S2O82-/O-2 system endowed the biosensor a high sensitivity. The linear range for ConA detection was from 0.0010 ng/mL to 10 ng/mL with a detection limit of 0.0002 ng/mL (S/N=3).
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