Toxic effects of diclofenac on life history parameters and the expression of detoxification-related genes in Daphnia magna

Diclofenac (DCF), as a widely used drug, has been detected in various environmental media such as municipal wastewater effluent. However, there is little information on the effects of DCF on freshwater invertebrates potentially exposing to its residues in surface water. In the present study, we investigated the toxic effects of DCF on the physiological parameters (e.g., survival, growth rate, and reproduction) of a crustacean, Daphnia magna, via a 21-d chronic toxicity test, and we also evaluated the effects of DCF on the expression of the genes related to the detoxification metabolism, growth, development and reproduction (e.g., HR96, P-gp, CYP360A8, CYP314, GST, EcR and Vtg) in acute exposure (up to 96 h) with RT-PCR. Significant toxic effects of DCF to D. magna were observed at 50 mu g L-1, the expression of these selected genes was inhibited with 24 h of exposure, and induced after 48 h to some extents. The expression of Vtg was induced at high concentrations of DCF (500 mu g L-1 and 5000 mu g L-1) after 24 h and 48 h of exposure, but also significantly induced at low concentration (50 mu g L-1) after 96 h of exposure. Dose and time-dependent relationships were observed for gene expression of the seven selected genes. In the 21-d chronic toxicity test, the days to the first brood and the days to the first egg production were both significantly delayed at 50 mu g L-1. However, there were no significant differences observed among the molting frequency, number of eggs produced in the first brood, total number of eggs per individual, total number of broods per individual, body length and intrinsic growth rate. Our results suggested that the reproduction parameters are more sensitive endpoints than the survival and growth for evaluating the toxicity of DCF to aquatic invertebrates. (C) 2016 Elsevier B.V. All rights reserved.