Journal
BIOMATERIALS
Volume 116, Issue -, Pages 48-56Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2016.11.031
Keywords
Skin-equivalent; Skin model; Vascular channel; Perfusion; Blood vessel; Percutaneous absorption
Funding
- Japan Society for the Promotion of Science (JSPS) [26-7064]
- Mazda Foundation
- JSPS [15K13904]
- Grants-in-Aid for Scientific Research [15K13904, 14J07064] Funding Source: KAKEN
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This paper describes a method for fabricating perfusable vascular channels coated with endothelial cells within a cultured skin-equivalent by fixing it to a culture device connected to an external pump and tubes. A histological analysis showed that vascular channels were constructed in the skin-equivalent, which showed a conventional dermal/epidermal morphology, and the endothelial cells formed tight junctions on the vascular channel wall. The barrier function of the skin-equivalent was also confirmed. Cell distribution analysis indicated that the vascular channels supplied nutrition to the skin-equivalent. Moreover, the feasibility of a skin-equivalent containing vascular channels as a model for studying vascular absorption was demonstrated by measuring test molecule permeation from the epidermal layer into the vascular channels. The results suggested that this skin-equivalent can be used for skin-on-a-chip applications including drug development, cosmetics testing, and studying skin biology. (C) 2016 Elsevier Ltd. All rights reserved.
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