Journal
BIORESOURCE TECHNOLOGY
Volume 225, Issue -, Pages 191-198Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2016.11.064
Keywords
Agave bagasse; Fuel ethanol; Ionic liquid; Organosolv Sequential saccharification and fermentation; Crystallinity
Funding
- Mexican National Council for Science and Technology (CONACYT) FONCICYT ERANet-LAC [C0013-248192]
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- bilateral international cooperation of SRE-AMEXCID (Mexico)
- Universidad Autonoma de Nayarit
- Programa Delfin
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Agave bagasse (AGB) has gained recognition as a drought-tolerant biofuel feedstock with high productivity in semiarid regions. A comparative analysis of ionic liquid (IL) and organosolv (OV) pretreatment technologies in AGB was performed using a sequential enzymatic saccharification and fermentation (SESF) strategy with cellulolytic enzymes and the ethanologenic Escherichia coli strain MS04. After pretreatment, 86% of xylan and 45% of lignin were removed from OV-AGB, whereas IL-AGB reduced lignin content by 28% and xylan by 50% when compared to the untreated biomass. High glucan (>90%) and xylan (>83%) conversion was obtained with both pretreated samples. During the fermentation stage (48 h), 12.1 and 12.7 kg of ethanol were produced per 100 kg of untreated AGB for IL and OV, respectively. These comparative analyses showed the advantages of SESF using IL and OV in a biorefinery configuration where a better understanding of AGB recalcitrance is key for future applications. (C) 2016 Elsevier Ltd. All rights reserved.
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