Canopy Chlorophyll Density Based Index for Estimating Nitrogen Status and Predicting Grain Yield in Rice

Canopy chlorophyll density (Chl) has a pivotal role in diagnosing crop growth and nutrition status. The purpose of this study was to develop Chl based models for estimating N status and predicting grain yield of rice (Oryza sativa L.) with Leaf area index (LAI) and Chlorophyll concentration of the upper leaves. Six field experiments were conducted in Jiangsu Province of East China during 2007, 2008, 2009, 2013, and 2014. Different N rates were applied to generate contrasting conditions of N availability in six Japonica cultivars (9915, 27123, Wuxiangjing 14, Wuyunjing 19, Yongyou 8, and Wuyunjing 24) and two Indica cultivars (Liangyoupei 9, YLiangyou 1). The SPAD values of the four uppermost leaves and LAI were measured from tillering to flowering growth stages. Two N indicators, leaf N accumulation (LNA) and plant N accumulation (PNA) were measured. The LAI estimated by LAI-2000 and LI-3050C were compared and calibrated with a conversion equation. A linear regression analysis showed significant relationships between Chl value and N indicators, the equations were as follows: PNA = (0.092 x Chl) - 1.179 (R-2 = 0.94, P < 0.001, relative root mean square error (RRMSE) = 0.196), LNA = (0.052 x Chl) - 0.269 (R-2 = 0.93, P < 0.001, RRMSE = 0.185). Standardized method was used to quantity the correlation between Chl value and grain yield, normalized yield = (0.601 x normalized Chl) + 0.400 (R-2 = 0.81, P < 0.001, RRMSE = 0.078). Independent experimental data also validated the use of Chl value to accurately estimate rice N status and predict grain yield.