Biotic and Abiotic Stresses Activate Different Ca2+ Permeable Channels in Arabidopsis

To survive, plants must respond rapidly and effectively to various stress factors, including biotic and abiotic stresses. Salinity stress triggers the increase of cytosolic free Ca2+ concentration ([Ca2+]) via Ca2+ influx across the plasma membrane, as well as bacterial flg22 and plant endogenous peptide Pep1. However, the interaction between abiotic stress-induced [Ca2+], increases and biotic stress-induced [Ca2+](i) increases is still not clear. Employing an aequorin-based Ca2+ imaging assay, in this work, we investigated the [Ca2+](i) changes in response to f1g22, Pep1, and NaCI treatments in Arabidopsis thaliana. We observed an additive effect on the [Ca2+](i) increase which induced by f1g22, Pep1, and NaCI. Our results indicate that biotic and abiotic stresses may activate different Ca2+ permeable channels. Further, calcium signal induced by biotic and abiotic stresses was independent in terms of spatial and temporal patterning.