Journal
ARCHIVES OF MEDICAL RESEARCH
Volume 48, Issue 2, Pages 169-179Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.arcmed.2017.03.014
Keywords
Jagged1; Notch3; Angiogenesis; Triple-negative breast cancer
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Funding
- National Natural Science Foundation of China [81502747]
- Youth Fund of Sichuan provincial people's hospital [30305030605]
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Background and Aims. Jagged1/Notch3 signaling pathway plays a key role in angiogenesis of breast cancer, but little is known in TNBC. This study was designed to investigate the expression of Jagged1/Notch3 mRNA and protein in TNBC, analyze their correlations with clinicopathological characteristics and prognosis. Moreover, the interrelationship among Jagged1/Notch3 and VEGF was initially evaluated. Methods. Jagged1/Notch3 mRNA and protein expression levels were determined by QRT-PCR and Western blotting. Additionally, Immunohistochemistry for Jagged1/Notch3 was detected by Ventana platform, VEGF and CD34 was performed using the EnVision/HRP technique. Results. mRNA transcriptionof Jagged1/Notch3 was in accord with protein expression. TNBC patients with positive Jagged1 expression had poorer DFS (p = 0.008) and OS (p = 0.004). Jagged1 expression was independent predictors of OS (p = 0.038). The expression of VEGF was positively correlative to MVD (p = 0.018), MVD was significantly associated with Jagged1 (p <0.0001) and Notch3 (p <0.0001). The expression of Jagged1/Notch3 has no correlation with VEGF, only in positive VEGF expression of TNBC patients Jagged1/Notch3 had influence on DFS and OS (p <0.05). Conclusion. Jagged1/Notch3 was -expressed at both the mRNA and protein levels, Jagged1 served as an independent predictor of poor prognosis. We speculate that there is a cross -talk between Jagged1/Notch3 and VEGF in TNBC angiogenesis. Jagged1/Notch3 is expected to be an important signaling pathway for TNBC progression and a potential target for TNBC neovascularization therapy. (C) 2017 IMSS. Published by Elsevier Inc.
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