Journal
FRONTIERS IN PLANT SCIENCE
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2017.01447
Keywords
Oryza sativa; regulated gene expression; XVE system; DNA recombination; seed-soaking
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Funding
- National Major Special Project on New Varieties Cultivation for Transgenic Organisms, China [2016ZX08001001-002]
- National Natural Science Foundation of China [U1405212, 31471760]
- Science and Technology Plan of Fujian Province, China [2014R1019-3]
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Inducible gene expression has emerged as a powerful tool for plant functional genomics. The estrogen receptor-based, chemical-inducible system XVE has been used in many plant species, but the limited systemic movement of inducer beta-estradiol in transgenic rice plants has prohibited a wide use of the XVE system in this important food crop. Here, we constructed an improved chemical-regulated, site-specific recombination system by employing the XVE transactivator in combination with a Cre/loxP-FRT system, and optimized a seed-soaking procedure for XVE induction in rice. By using a gus gene and an hpRNAi cassette targeted for OsPDS as reporters, we demonstrated that soaking transgenic seeds with estradiol solution could induce highly efficient sitespecific recombination in germinating embryos, resulting in constitutive and high-level expression of target gene or RNAi cassette in intact rice plants from induced seeds. The strategy reported here thereby provides a useful gene activation approach for effectively regulating gene expression in rice.
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