Journal
ACS NANO
Volume 11, Issue 2, Pages 2052-2059Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.6b08232
Keywords
iridium nanoparticles; gold vesicle; enzyme mimic; detection; biomarker
Categories
Funding
- National Science Foundation (NSF) Career Award [CHE-1651307]
- Michigan Technological University (MTU)
- NSF Career Award [DMR-1255377]
- U.S. Department of Energy, Basic Energy Sciences, Division of Materials Science and Engineering [DE-AC02-98CH10886]
- Division Of Materials Research
- Direct For Mathematical & Physical Scien [1255377] Funding Source: National Science Foundation
Ask authors/readers for more resources
Enzyme-based colorimetric assays have been widely used in research laboratories and clinical diagnosis for decades. Nevertheless, as constrained by the performance of enzymes, their detection sensitivity has not been substantially improved in recent years, which inhibits many critical applications such as early detection of cancers. In this work, we demonstrate an enzyme-free signal amplification technique, based on gold vesicles encapsulated with Pd-Ir nanoparticles as peroxidase mimics, for colorimetric assay of disease biomarkers with significantly enhanced sensitivity. This technique overcomes the intrinsic limitations of enzymes, thanks to the superior catalytic efficiency of peroxidase mimics and the efficient loading and release of these mimics. Using human prostate surface antigen as a model biomarker, we demonstrated that the enzyme-free assay could reach a limit of detection at the femtogram/mL level, which is over 10(3)-fold lower than that of conventional enzyme-based assay when the same antibodies and similar procedure were used.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available