Journal
FRONTIERS IN PLANT SCIENCE
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fpls.2017.01287
Keywords
Arabidopsis thaliana; chloroplast; embryogenesis; storage reserves; oil body; protein storage body
Categories
Funding
- National Natural Science Foundation of China [31371387]
- Beijing Municipal Commission of Education for Training plan of Young Talents [CIT TCD 201504081]
Ask authors/readers for more resources
The chloroplast is one of the most important organelles found exclusively in plant and algal cells. Previous reports indicated that the chloroplast is involved in plant embryogenesis, but the role of the organelle during embryo morphogenesis and maturation is still a controversial question demanding further research. In the present study, siliques of Arabidopsis at the early globular stage were enwrapped using tinfoil, and light deprivation-induced inhibition of the chloroplast biogenesis were validated by stereomicroscope, laser scanning confocal microscope and transmission electron microscope. Besides, the effects of inhibited chloroplast differentiation on embryogenesis, especially on the reserve deposition were analyzed using periodic acidSchiff reaction, Nile red labeling, and Coomassie brilliant blue staining. Our results indicated that tinfoil enwrapping strongly inhibited the formation of chloroplasts, which did not arrest embryo morphogenesis, but markedly influenced embryo maturation, mainly through reducing the accumulation of storage reserves, especially starch grains and oil. Our data provide a new insight into the roles of the chloroplast during embryogenesis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available