Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 3, Pages 2039-2048Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b04632
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Funding
- National Natural Science Foundation of China [21475099, 21535005]
- 863 Program [2013AA032204]
- National Science and Technology Major Project [2016ZX10004222]
- Natural Science Foundation of Hubei [2014CFA003]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences (CAS)
- Canadian Institutes of Health Research (CIHR)
- President's International Fellowship Initiative from the CAS
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The 2014-16 Ebola virus (EBOV) outbreak in West Africa has attracted widespread concern. Rapid and sensitive detection methods are urgently needed for diagnosis and treatment of the disease. Here, we propose a novel method for EBOV detection based on efficient amplification of electroluminescent nanospheres (ENs) coupled with immunomagnetic separation. Uniform ENs are made by embedding abundant amounts of CdSe/ZnS quantum dots (QDs) into copolymer nanospheres through simple ultrasound. Compared to QDs, ENs can enhance electroluminescence (ECL) signals by approximately 85 fold, achieving a signal to background ratio high enough for EBOV detection. The introduction of magnetic nanobeads (MBs) can selectively separate targets from complex samples, simplifying the operation process and saving time. The presence of MBs can amplify ECL by approximately 3 fold, improving detection sensitivity. By integration of ENs with MBs, a sensitive electroluminescence biosensor is established for EBOV detection. The linear range is 0.02-30 ng/mL with a detection limit of 5.2 pg/mL. This method provides consistent reproducibility, specificity, and anti interference ability and is highly promising in clinical diagnosis applications.
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