Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 3, Pages 1439-1444Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b04587
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Funding
- Pioneer Research Center Program through the National Research Foundation (NRF) of Korea - Ministry of Science, ICT & Future Planning (MSIP) [2013M3C1A3064777]
- NRF grant - Korea government (MSIP) [2015R1C1A1A01053990, 2014R1A2A2A09052449]
- National Research Foundation of Korea [2015R1C1A1A01053990] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Blood plasma separation from whole blood is often limited by numerous blood cells which can compromise separation processes and thus deteriorate separation performance such as purity and throughput. To address this challenge, we present a microfluidic pipet tip composed of slant array ridges that enable autonomous blood cell focusing without significant deviation as well as facilitating a high degree of parallelization without compromising separation purity. With these advantages, we achieved high-purity (99.88%) and high-throughput (904.3 mu L min(-1) ) plasma separation from whole blood. In combination with a smart pipet, we successfully demonstrated rapid, inexpensive, and equipment-free blood plasma preparation for pretransfusion testing.
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