Identification, Bioactivity, and Productivity of Actinomycins from the Marine-Derived Streptomyces heliomycini

In the process of profiling the secondary metabolites of actinobacteria isolated from the Saudi coastal habitats for production of antibiotics and anti-cancer drugs, the cultures of strain WH1 that was identified as Streptomyces heliomycini exhibited strong antibacterial activity against Staphylococcus aureus. By means of MS and NMR techniques, the active compounds were characterized as actinomycins X-0 beta, X-2, and D, respectively. The research on the productivity of this strain for actinomycins revealed that the highest production of actinomycins X-0 beta, X-2. and D was reached in the medium Mil within 5% salinity and pH 8.5. In this optimized condition, the fermentation titers of actinomycins X-0 beta, X-2, and D were 107.6 +/- 4.2, 283.4 +/- 75.3, and 458.0 +/- 76.3 mg/L, respectively. All the three actinomycins X-0 beta, X-2, and D showed potent cytotoxicities against the MCF-7, K562, and A549 tumor cell lines, in which actinomycin X-2 was the most active against the three tumor cell lines with the IC50 values of 0.8-1.8 nM. Both actinomycins X-2 and D showed potent antibacterial activities against S. aureus and the methicillin-resistant S. aureus, Bacillus subtilis, and B. cereus and the actinomycin X-2 was more potent.