Journal
CHEMICAL COMMUNICATIONS
Volume 53, Issue 13, Pages 2182-2185Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6cc09934c
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Funding
- Herchel Smith Chair of Biochemistry Fund
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- BBSRC [BB/J007250/1]
- EPSRC
- Biotechnology and Biological Sciences Research Council [BB/J007250/1] Funding Source: researchfish
- BBSRC [BB/J007250/1] Funding Source: UKRI
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Following the in vivo investigation of thiotetronate assembly in Lentzea sp. and in S. thiolactonus NRRL 15439 (Havemann et al., Chem. Commun., 2017, DOI: 10.1039/c6cc09933e), the minimal set of genes required for thiolactomycin production was determined through heterologous expression and the mechanism for polyketide assembly was established in vitro through incubation of recombinant TlmB with its substrates in the presence of either nonhydrolysable or hydrolysable chemical probes. The results presented here constitute unequivocal evidence of enzymatic processing by an unusual iterative polyketide synthase.
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