Journal
ANALYTICAL CHEMISTRY
Volume 89, Issue 2, Pages 1373-1381Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b04840
Keywords
-
Categories
Funding
- National Science Foundation, Chemistry Division [CHE-1306928]
- National Cancer Institute of the NIH [1R33CA206955-01]
- PhageTech Inc [PHAGE-203015]
- Chao Family Comprehensive Cancer Center, UC Irvine
Ask authors/readers for more resources
The label-free detection of human serum albumin (HSA) in aqueous buffer is demonstrated using a simple, monolithic, two-electrode electrochemical biosensor. In this device, both millimeter-scale electrodes are coated with a thin layer of a composite containing M13 virus particles and the electronically conductive polymer poly(3,4-ethylenedioxy thiophene) or PEDOT. These virus particles, engineered to selectively bind HSA, serve as receptors in this biosensor. The resistance component of the electrical impedance, Z(re), measured between these two electrodes provides electrical transduction of HSA binding to the virus-PEDOT film. The analysis of sample volumes as small as 50 mu L is made possible using a microfluidic cell. Upon exposure to HSA, virus-PEDOT films show a prompt increase in Z(re) within 5 s and a stable Zre signal within 15 min. HSA concentrations in the range from 100 nM to 5 mu M are detectable. Sensor-to-sensor reproducibility of the HSA measurement is characterized by a coefficient-of-variance (COV) ranging from 2% to 8% across this entire concentration range. In addition, virus-PEDOT sensors successfully detected HSA in synthetic urine solutions. ,
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available