Journal
DALTON TRANSACTIONS
Volume 46, Issue 7, Pages 2159-2164Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c6dt04443c
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Funding
- Swiss National Science Foundation [PP00P2_144700]
- Swiss National Science Foundation through the National Centre of Competence in Research Bio- Inspired Materials
- Adolphe Merkle Foundation
- Swiss National Science Foundation (SNF) [PP00P2_144700] Funding Source: Swiss National Science Foundation (SNF)
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Herein we report the synthesis of new water-soluble vitamin B-12 prodrugs bearing metal complexes at the beta-upper side of the cobalt center. A total of three derivatives with the general design {Co-C C-bpy-M}, where M represents a cytotoxic metal complex, were prepared and tested for their cytotoxicity against MCF-7 breast cancer cells. The choice of the metal was oriented on the eminent Pt and promising Ru and Re species to demonstrate the general applicability of the approach. The recognition of the derivatives by transcobalamin was demonstrated by competitive displacement assays using rhodamine labeled B-12. This compound further served to prepare a dual luminescent probe by orthogonal synthesis with M = ((HCCbpy) Ru(bpy)(2)) Cl-2 and to perform in vitro assays. Cellular imaging experiments allowed us to observe the different compartmentalization of both dyes and thus prove that the species follow the natural cobalamin uptake as well as the self-triggered release of the beta-upper complex.
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