Journal
ELIFE
Volume 6, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.21926
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Categories
Funding
- Wellcome [WT093736]
- Biotechnology and Biological Sciences Research Council [BB/J004480/1]
- Medical Research Council [MR/J003808/1]
- BBSRC [BBS/E/B/000C0405, BBS/E/B/000C0404, BBS/E/B/0000H331, BBS/E/B/000C0421, BB/M022285/1, BBS/E/B/000C0422, BBS/E/B/000C0400, BBS/E/B/000C0402] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BBS/E/B/000C0405, BBS/E/B/000C0402, BBS/E/B/000C0421, BB/M022285/1, BBS/E/B/000C0400, BBS/E/B/000C0422, BBS/E/B/000C0404, BBS/E/B/0000H331] Funding Source: researchfish
- Medical Research Council [1520383, MC_PC_12009] Funding Source: researchfish
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Long-range cis-regulatory elements such as enhancers coordinate cell-specific transcriptional programmes by engaging in DNA looping interactions with target promoters. Deciphering the interplay between the promoter connectivity and activity of cis-regulatory elements during lineage commitment is crucial for understanding developmental transcriptional control. Here, we use Promoter Capture Hi-C to generate a high-resolution atlas of chromosomal interactions involving similar to 22,000 gene promoters in human pluripotent and lineage-committed cells, identifying putative target genes for known and predicted enhancer elements. We reveal extensive dynamics of cis-regulatory contacts upon lineage commitment, including the acquisition and loss of promoter interactions. This spatial rewiring occurs preferentially with predicted changes in the activity of cis-regulatory elements and is associated with changes in target gene expression. Our results provide a global and integrated view of promoter interactome dynamics during lineage commitment of human pluripotent cells.
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